The cellulases endoglucanase I and cellobiohydrolase II of Trichoderma reesei act synergistically to solubilize native cotton cellulose but not to decrease its molecular size
Article Abstract:
Enzymatic hydrolysis studies on degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) demonstrate synergy between EGI and CBHII in inhibiting sugar production, with EGI serving as a prominent degrading factor. Size exclusion chromatography of the tricarbanilate derivatives reveals no change in the molecular-size distribution of cellulose in the post-degradation phase. EGI selectively cleaves through the microfibrils, whereas CBHII releases soluble sugars from the degraded portions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Exploration of new geometries in cellulosome-like chimeras
Article Abstract:
Novel cellulosome chimeras displaying atypical geometries and binding modes are designed, wherein the targeting and proximity functions are directly incorporated as integral parts of the enzyme components. The analysis has shown that cellulosome components are used for generating higher-order functional composites and has suggested that enzyme mobility is a key parameter for cellulosome efficiency.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Two-dimensional crystallography of TFIIB- and IIE-RNA polymerase II complexes: implications for start site selection and initiation complex formation
- Abstracts: Erosion from Staphylococcus aureus biofilms grown under physiologically relevant fluid shear forces yields bacterial cells with reduced avidity to collagen
- Abstracts: Connecting two-component regulatory systems by a protein that protects a response regulator from dephosphorylation by its cognate sensor
- Abstracts: Normal levels of wild-type mitochondrial DNA maintain cytochrome c oxidase activity for two pathogenic mitochondrial DNA mutations but not for m.3243A->G. part 2
- Abstracts: Ralstonia solanacearum pectin methylesterase is required for growth on methylated pectin but not for bacterial wilt virulence