The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events
Article Abstract:
The sequential formation of stacks of active cisternae from vesiculated Golgi membranes (VGMs) is controlled by the ATPases a N-ethylmalemide-sensitive factor (NSF) and p97, a NSF-like ATPase. The first step in the formation of the cisternae during which the dispersed small VGMs fuse to form larger vesicles is controlled by NSF, and the subsequent fusion of these vesicles to form tubules and cisternae is controlled by p97. Although the mechanism of stack formation is similar to that of fusion of transport vesicles, inhibitors of vesicle transport do not inhibit stack formation.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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Subtype-specific and ER lumenal environment-dependent regulation of inositol 1,4,5-trisphosphate receptor type 1 by ERp44
Article Abstract:
An endoplasmic reticulum (ER) lumenal protein of the thioredoxin family, ERp44, directly interacts with the third lumenal loop of inositol 1,4,5-trisphosphate receptor (IP3R) type 1(IP3R1) and the interaction is dependant on pH, Ca2+ concentration, and redox state. Thus ERp44 senses the environment in the ER lumen and modulates IP3R1 activity, contributing to regulating both intralumenal conditions and the complex patterns of cytosolic Ca(super2+) concentrations.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
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Golgi membranes remain segregated from the endoplasmic reticulum during mitosis in mammalian cells
Article Abstract:
A procedure to address whether Golgi membranes combine with the Endoplasmic Reticulum (ER) during mitosis via the detection of interactions between ER and Golgi proteins are developed. This procedure depends on the ability of two proteins to conditionally bind with the presence of ligand. Rapamycin, a small molecule, binds to the FK506 binding protein (FKBP).
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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