The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration
Article Abstract:
The pNP40 lactococcal plasmid encodes an early acting mechanism which confers resistance to the phi-c2 phage by preventing phage DNA penetration. However, electroporation of the phage DNA into resistant bacteria increases the formation of phage progeny in the cells as compared to traditionally infected cells. The resistance is specific for phi-c2 phage and has no effect on the phi-712 phage. The resistance mechanism is different from the adsorption inhibition, restriction and modification, and abortive infection resistance mechanisms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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AbiG, a genotypically novel abortive infection mechanism encoded by plasmid pCI750 of Lactococcus lactis subsp. cremoris UC653
Article Abstract:
The pCI750 conjugated plasmid of Lactococcus lactis subsp. cremoris UC653 encodes AbiG, an abortive infection mechanism that fails to suppress phage DNA replication. AbiG makes the bacteria completely resistant to phi-712 and partially resistant to phi-c2. The absence of pCI750 DNA helps in the expression of the resistance mechanism. The AbiG gene locus contains two open reading frames, abiGi and abiGii, which show insignificant homology to the sequences of other DNAs and proteins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Application and evaluation of the phage resistance- and bacteriocin-encoding plasmid pMRC01 for the improvement of dairy starter cultures
Article Abstract:
The conjugative 63-kb lactococcal plasmid pMRC01 was transferred into several lactococcal starter cultures to enhance their phage resistance characteristics. Findings showed that efficient transfer of the plasmid is dependent on the particular recipient strain selected. Also, while there was high frequency transfer of the entire plasmid to some strains, the plasmid could not be conjugated into a number of starters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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