The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundaries
Article Abstract:
The two dimension gel patterns of replication intermediates from several variants in which parts or all of the DHFR promoter is deleted is examined to test whether transcription through the DHFR gene is required to activate this origin in early S phase. The findings indicate that transcription through the gene not only suppresses initiation in the body of the gene but also helps to define the boundaries of the downstream origin.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication
Article Abstract:
The Chinese hamster dihydrofolate reductase (DHFR) origin of replication consists of a 55-kb zone of potential initiation sites lying between the convergently transcribed DHFR and 2BE2121 genes. Studies conclude that the normal 3' end of the DHFR gene constitutes a boundary element not only for the gene but also for the local origin of replication.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression
Article Abstract:
Protein Sum 1 is a novel heterochromatin-targeting factor for HML that acts as a repressor of meiotic genes during vegetative growth. Sum 1 is required for origin activity of HML-E and is useful for regulating replication initiation.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The polarisation of the anteroposterior axis in Drosophila. The formation of sense organs in Drosophila: a logical approach
- Abstracts: Elucidation of the hrp clusters of Xanthomonas oryzae pv. oryzicola that control the hypersensitive response in nonhost tobacco and pathogenicity in susceptible host rice
- Abstracts: Multiple branches of the meiotic recombination pathway contribute independently to homolog pairing and stable juxtaposition during meiosis in budding yeast
- Abstracts: Differential gene regulation by the SRC family of coactivators. NIR is a novel INHAT repressor that modulates the transcriptional activity of p53
- Abstracts: Muscle stem cells in development, regeneration, and disease. WUSCHEL induces shoot stem cell activity and developmental plasticity in the root meristem