The response of selected members of the archaea to the Gram stain

Article Abstract:

Gram staining is a technique in microscopy which leads to the classification of new isolates of bacteria as either Gram-positive or Gram-negative. The results is based on the reaction of the stain with the cell envelopes. Application of this technique on five members of the archaea, which possess a broad range of cell envelope structural formats, classified Methanobacterium formicicum as Gram-positive, suggesting that its cell walls are overlying the cell membrane. Methanospirillum hungatei also stained Gram-positive but only because the stain gained entry to its terminal filament cells. Methanosarcina mazei, Methanococcus jannaschii and Sulfolobus acidocaldarius stained Gram-negative because of their thick cell walls.

Analysis, Usage, Stains and staining (Microscopy), Stains (Microscopy), Bacterial cell walls, Archaeabacteria, Archaea

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Organization of carbamoyl-phosphate synthase genes in Neisseria gonorrhoeae includes a large, variable intergenic sequence which is also present in other Neisseria species

Article Abstract:

The DNA sequence coding for the synthesis of carbamoyl-phosphate synthase in Neisseria gonorrhoeae, carA and carB, which are found to be separated by a lengthy distance of 3290 base pairs. These sequences contain repetitive sequences. The long intervening sequence was found to vary in length within a range of 2200 to 3700 base pairs. This was compared to the intervening sequence in N. meningitis, which showed a similar variation and lenght.

Research, Enzymes, Nucleotide sequence, Base sequence

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Deletion of the cell-division inhibitor MinC results in lysis of Neisseria gonorrhoeae

Article Abstract:

Deletion of the cell-division inhibitor MinC has been found to result in lysis of Neisseria gonorrhoeae. The minCDE genes involved in division site selection in N. gonorrhoeae were identified using raw data from the N. gonorrhoeae genome project and are part of a cluster of 27 genes. The role of MinC is necessary for maintaining proper division in cocci.

United States, Statistical Data Included, Physiological aspects, Coccidia, Cryptosporidium, Cytochemistry, Cell division, Neisseria gonorrhoeae, Lysogeny

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