The thyA gene from Bacillus subtilis exhibits similarity with the phage phi-3T thymidylate synthase gene
Article Abstract:
The thymidylate synthase A-encoding thyA gene that is cloned from Bacillus subtilis 168 genomic library exhibits sequence similarity with phage phi3T thymidylate synthase gene. The sequence similarity is restricted to the coding region and the neighboring 3' region that cover 862 nucleotides, with the flanking sequences being different between the two genes. Replacement of chromosomal thyA gene with an interactive plasmid that contains a deletion in the thyA region yields transformants that exhibit growth impairment at 47 degree Celsius in the absence of thymidine. Genetic and physical mapping techniques indicate that thyA maps to 168 degree on the B. subtilis chromosome.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtils: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase
Article Abstract:
PCR amplification of the extracellular xylanase-encoding xyn A gene results in a 770 bp DNA fragment from Bacillus subtilis 168 chromosome DNA. A search of the B. subtilis 168 genomic library yields the eglS gene that codes for endo-beta-1,4-glucanase and the bglS gene that codes for endo-beta-1,3-1,4-glucanase. Substitution of chromosomal eglS, bglS and xynA gene of B. subtilis 168 with integrative plasmids with a coding region deletion-containing DNA fragment yields strains that lack xylan and carboxymethylcellulose activity. eglS gene maps to 170 degrees, while xynA maps to 175 degrees and bglS maps to 340 degrees on the chromosome.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: a region devoted to purine uptake and metabolism, and containing the genes cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide sequence
Article Abstract:
The complete sequencing of the 52 degrees-55 degrees segment of the Bacillus subtilis choromosome which contains a pur operon, 17 putative open reading frames (ORF) and a partial ORF. The 34960bp contig. is composed of cotA, gabP and guaA genes together with the genes of the pur operon. The cotA gene was located at the 52 degrees segment and exhibits a molecular mass of of 58 kDa. The gabP gene is composed of 12 transmembrane helices and acts a monocitronic transcript that encodes the gamma-aminobutyrate permease.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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- Abstracts: Novel methods for genetic transformation of natural Bacillus subtilis isolates used to study the regulation of the mycosubtilin and surfactin synthetases
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