Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase, which fails to be synthesized in an active form in Escherichia coli
Article Abstract:
The genes, which encode Mn-dependent catalases from Thermus thermophilus and HB8 and a mutant, which is not so thermostable but carries two amino acid replacements, (M129V and E293G) were all combined together. In the Escherichia coli, if the wild-type and mutant genes are appearing in excess then the unchanged or six-his-tagged proteins of the anticipated size are created in large numbers as inactive proteins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Examination of the watershed-wide distribution of Escherichia coli along Southern Lake Michigan: An integrated approach
Article Abstract:
The interrelatedness of Escherichia coli occurrence from various coastal watershed components along southern Lake Michigan is examined. Results reveal that once established in forest soil, E. coli could persist throughout the year, potentially acting as a continuous non-point source of E. coli to nearby streams.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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