Three-dimensional localization of wild-type and myosin II mutant cells during morphogenesis of Dictyostelium
Article Abstract:
Individual Dictyostelium amoebae can be localized and tracked at high resolution to study development within the multicellular organism, using a fluorescent dye for labelling. The technique has been used to determine why mutant amoebae without myosin II fail to accomplish morphogenesis. Labeled wild-type cells are randomly distributed in the organism and finish normal development. Mutant cells without cortical integrity are apparently unable to break the adhesion of wild-type cells, indicating that mutants lacking myosin II are unable to complete morphogenesis, perhaps because of their inability to move effectively within the mass of adhered cells.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
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Involvement of cyanide-resistant respiration in cell-type proportioning during Dictyostelium development
Article Abstract:
Migrating slugs were stained with rhodamine 123, which stains actively respiring mitochondria, and treated with benzohydroxamic acid (BHAM) and propyl gallate, which inhibit cyanide (CN)-resistant respiration, to induce production of unique cell masses. Cells strongly expressed prestalk-specific genes in a position-dependent manner, but showed reduced expression of prespore-specific genes. These results suggest that cell differentiation is strongly correlated with CN-resistant respiration during slug development.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
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3D analysis of cell movement during normal and myosin-II-null cell morphogenesis in dictyostelium
Article Abstract:
Examination of the three-dimensional trajectories of both normal axenic and myosin-II-null cells of Dictyostelium discoideum amebae by time-lapse computational optical-sectioning microscopy reveals that three-dimensional ameboid locomotion requires myosin-II. Locomotion of the amebae is regulated by various distinct, potentially competing cell-guide mechanisms.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
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