Two different Bacillus thuringiensis delta-endotoxin receptors in the midgut brush border membrane of the European corn borer, Ostrinia nubilalis (Hubner)(Lepidoptera: Pyralidae)
Article Abstract:
The presence of insecticidal crystal protein (ICP) receptors in the European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Pyralidae), the major lepidopteran pest of maize in southern Europe and North America, is studied. Two independent ICP receptors are present in the brush border of O.nubilalis gut epithelium. The interactions of B-thuringiensis ICPs with the larval gut of O.nubilalis is investigated by the experiments performed to determine binding of ICPs to both isolated brush border membrane vesicles and tissue sections.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Biotinylation of Bacillus thuringiensis insecticidal crystal proteins
Article Abstract:
The usefulness of Bacillus thuringiensis insecticidal crystal protein (ICP) is studied. An alternative method is established by it for the screening of novel ICPs toxic to pest insects, and for ICP receptor characterization. The results of heterologous competition experiments performed with biotinylated and native ICPs confirms the relationship between different ICP receptor types in Plutella xylostella which is inferred from radioligand binding studies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Analyses of Cry1Ab binding in resistant and susceptible strains of the European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae)
Article Abstract:
Cry1Ab toxin binding analysis was conducted to find out if altered binding is associated with Cry1Ab resistance in selected Ostrinia (O.) nubillalis strains. The results show that differences in susceptibility to Cry1A toxins in Europe-R strain of O. nubilalis are associated with altered receptor binding, though the exact nature of this mechanism is unclear.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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