Use of PMAI as a housekeeping biomarker for assessment of toxic-induced stress in Saccharomyces cerevisiae
Article Abstract:
Toxicant induced stress of pure compounds in Saccharomyces cerevisiae utilizing a destabilized version of green fluorescent protein optimized for expression in yeast (yEGFP3) under control of the promoter of the housekeeping membrane ATPease gene PMAI were studied. It demonstrated the versatility of the novel biomarker to complement existing test batteries to assess contaminant exposure and effects.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Effects of ADH2 overexpression in Saccharomyces bayanus during alcoholic fermentation
Article Abstract:
The effect of overexpression of the gene ADH2 on metabolic and biological activity in Saccharomyces bayanus V5 during alcoholic fermentation is examined. The increased contents in acetoin, acetic acid, and L-proline present in the fermentation medium is attributed to detoxification by removal of excess acetaldehyde and the need to restore and maintain the intracellular redox potential balance.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
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Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations
Article Abstract:
The transferability of this respiratory phenotype to the V5hxt1-7delta strain a derivative of a strain used in enology was demonstrated. Even the more relevant for the potential use of such a strain for the production of heterologous protein and also of low-alcohol beverages is the observation that the biomass yield increased 50% with the mutant compared to its parental strain.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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