Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes
Article Abstract:
A test using PCR and a fluorogenic probe was used to count populations of the bacterium Listeria (L.) monocytogenes and may be useful in detecting other bacteria that contaminate food and cause disease. The assay uses a Taq DNA polymerase and a probe that is linked to two fluorescent dyes. When the probe is hydrolyzed the intensity of the fluorescence increases, indicating the amount of PCR product. With L. monocytogenes, the test took three hours and could detect up to 50 CFU.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Polymerase chain reaction amplification of napthalene catabolic and 16S rRNA gene sequences from indigenous sediment bacteria
Article Abstract:
The utilization of polymerase chain reaction (PCR) to amplify bacterial genes from uninoculated surface and subsurface sediments is discussed. Bacterial strains for the experiment were drived from Pseudomonas putida and was subjected to a napthalene-contaminated field site. DNA extraction with gel electrophoresis was used to render the bacteria amplifiable by PCR. The utility of PCR for studies on function- and taxon specific DNA was established.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Horizontal transfer of phnAc dioxygenase genes within one of two phenotypically and genotypically distinctive naphthalene-degrading guilds from adjacent soil environments
Article Abstract:
The horizontal transfer of the phnAc sequence is indicated among the hillside naphthalene-degrading isolates from a polycyclic aromatic hydrocarbon-contaminated soil as shown by 16S ribosomal DNA sequence analysis. Furthermore, these isolates exhibit both genetic and metabolic diversity, suggesting their acclimation to pollutants.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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