Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila

Article Abstract:

The production of germline chimeras has usually involved the use of x-ray irradiation to recover germline mosaics in individuals heterozygous for dominant female sterile (DFS) mutations. This technique gives low frequencies of recovery of mosaic females. However, by using the yeast recombinase, FLP, to induce site-specific homologous mitotic recombination in flies heterozygous for a DFS mutation, more that 90% of mosaic females are recovered. This technique, termed the 'FLP-DFS' technique, was used to analyse the maternal effect phenotypes of a large number of X-linked zygotic lethal mutations.

Methods, Genetic engineering, Drosophila

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Role of Erf recombinase in P22-mediated plasmid transduction

Article Abstract:

Plasmid transduction can be achieved in Salmonella typhimurium by using phage P22 mutants. Plasmids that possessed regions homologous to the P22 genome exhibited efficient transfer for wild-type P22. The efficient transduction is achieved when a plasmid-phage cointegrate replicates or when a high-transducing mutant system recognizes a plasmid nucleotide sequence as a pac-like site. Although Erf recombinase can sponsor plasmid molecule reconstruction, it does not display a similar function for chromosomal molecules.

Plasmids, Bacteriophages, Salmonella typhimurium, Transduction, Transduction (Genetics)

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Gene dosage analysis in Azotobacter vinelandii

Article Abstract:

A study was conducted to analyze the identity of Azotobacter vinelandii as a polyploid bacterium. Earlier findings based on chromosome size and DNA content per cell indicated such an identity for Azotobacter vinelandii. However, genetic operations performed on it indicate noncompliance with the nature of polyploid bacteria. These operations include chromosomal lac fusions and delay analysis involving mutations. Results of these tests show that Azotobacter vinelandii is not a polyploid bacterium.

Analysis, Bacterial genetics, Microbial mutation, Polyploidy

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