Use of antibacterial agents to elucidate the etiology of juvenile oyster disease (JOD) in Crassostrea virginica and numerical dominance of an alpha-proteobacterium in JOD-affected animals
Article Abstract:
Juvenile oyster disease (JOD) has resulted in high seasonal losses of cultured Eastern oysters since 1988. The antibacterial antibiotics norfloxacin and sulfadimethoxine-ormetoprim were tested for the ability to delay the onset of JOD mortality and/or reduce the JOD mortality of cultured juvenile Crassostrea virginica. Bacteriological analyses revealed a numerically dominant bacterium in the animals with obvious signs of JOD. Sequence analysis of the 16S rRNA gene from these bacteria indicates that they are a previously undescribed species of marine alpha-proteobacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Overcoming a defect in generalized recombination in the marine fish pathogen Vibrio anguillarum 775: construction of a recA mutant by marker exchange
Article Abstract:
A Vibrio anguillarum H775-3 recA mutant was constructed by a modified marker exchange technique. Specific mutations were introduced by inserting large segments of homologous DNA carrying the recA gene of V. anguillarum 775 in the chromosomal DNA of V. anguillarum H775-3 thru conjugal mobilization. Unlike its parent, the recA mutant exhibited recombination, suggesting that recA is required for DNA repair in V. anguillarum 775.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Additional evidence that juvenile oyster disease is caused by a member of the Roseobacter group and colonization of nonaffected animals by Stappia stellulata-like strains
Article Abstract:
Results demonstrate that species of the alpha-proteobacteria of the Roseobacter group (CVSP) are the only etiologic agent of the juvenile oyster disease (JOD) epizootic. Animals not exposed to JOD are colonized by Stappia stellulata-like strains. JOD was reproduced under laboratory conditions by exposing Crassostrea virginica cells to CVSP bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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