Use of high-affinity cell wall-binding domain of bacteriophage endolysins for immobilization and separation of bacterial cells

Article Abstract:

A novel concept is described for the immobilization and separation of bacterial cells by replacing antibodies with cell wall-binding domains (CBDs) of bacteriophage-encoded peptidoglycan hydrolases (endolysins). The observation has showed that CBD polypeptides represent novel and innovative tools for the binding and capture of bacterial cells with many possible applications in microbiology and diagnostics.

Science & research, Bacteriophages, Peptidoglycans, Bacterial cell walls

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Long-chain polyphosphate causes cell lysis and inhibits Bacillus cereus septum formation, which is dependent on divalent cations

Article Abstract:

Research was conducted to examine the cellular mechanisms leading to morphological shifts, growth inhibition and lysis of Bacillus cereus WSBC 10030 when challenged with a long-chain polyphosphate (polyP). The objective was to analyze the mechanism of polyP inhibition in greater detail. Results indicate that polyP indirectly influences the general division mechanism of cells, the Fts protein family.

Cations, Bacterial proteins, Bacillus cereus, Septum (Brain)

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