Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification
Article Abstract:
Rolling circle amplification (RCA) technique is applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coil cells carrying a specific gene sequence. This method can become a valuable tool for studying free DNA uptake, especially in nonculturable bacteria, by optimizing the cell permeabilization condition for in situ RCA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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rRNA sequence-based scanning electron microscopic detection of bacteria
Article Abstract:
A new scanning electron microscopic method was developed for gaining both phylogenetic and morphological information about target microbes using in situ hybridization with rRNA-targeted oligonucleotide probes. Target cells were hybridized with oligonucleotide probes after gold labeling.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
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