Xanthobacter flavus employs a single triosephosphate isomerase for heterotrophic and autotrophic metabolism

Article Abstract:

Mutants were isolated with an altered regulation of the promoter of the cbb operon to identify the components involved in the regulation of the Calvin cycle. This isolation led to the identification of a pgk mutation which caused increased repression of the cbb promoter by gluconeogenic substrates that prevented autotrophic growth. Furthermore, cell extracts of the mutant grown under glycolytic or gluconeogenic conditions had severely reduced triosephosphate isomerase activities.

Mutation (Biology), Mutation, Isomerases

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Structure of asparagine-linked oligosaccharides of an aspartic proteinase from the zygomycete fungus Rhizomucor pusillus

Article Abstract:

The high-mannose oligosaccharide moieties linked to two asparagine (Asn) residues of the aspartic proteinase secreted by Rhizomucor pusillus at positions 79 and 188 were analyzed using high-performance liquid chromatography after cleavage from the enzyme and tagging with a fluorescent dye. The moiety attached to Asn79 is composed of five mannose (Man) and two N-acetyl-glucoseamine (GlcNAc) residues while that attached to Asn188 contained five to six Man and two GlcNAc residues.

Oligosaccharides, Microbial polysaccharides, Microbial enzymes

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An operon encoding aspartokinase and purine phosphoribosyltransferase in Thermus flavus

Article Abstract:

Two operon replicating factors (ORF) that coded for a phosphoribosyltransferase-like protein as well as the genes for encoding aspartokinases were found side by side in the chromosome of Thermus flavus. The first ORF, which encodes aspartokinase, overlaps with the next ORF, and both are transcribed by only one messenger RNA. Both are similar to the genes that regulate the thermal stability of aspartokinase and phsophorbosyltransferase in E. coli.

Genetic aspects, Enzymes

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