undulated phenotypes suggest a role of Pax-1 for the development of vertebral and extravertebral structures

Article Abstract:

Removal of the terminal Pax-1 exon including the poly(A) signal leads to a 28.2 kb deletion in undulated extensive (un(ex)) mice, resulting in very low Pax-1 transcripts. Comparative phenotype analysis of un(ex), undulated short tail and undulated alleles indicates that the facial skeleton, verterbral column, scapula and sternum undergo morphological variations that are related to Pax-1 expression regions. Delayed cell accumulation in the embryonic sclerotome leads to reductions in the vertebral components.

Analysis, Growth, Vertebrae, Animal mutation

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Developmental analysis of the Hba superscript th-J mouse mutation: effects on mouse peri-implantation development and identification of two candidate genes

Article Abstract:

A polymerase chain reaction (PCR)-based test was used to identify and characterize the genotype of individual embryos homozygous for the Hba superscript th-J deletion in mutant mice. Southern blotting was used to determine whether the transcripts of genes mapped within the upstream region of the Hba locus are expressed in different stages of development. Reverse-transcriptase PCR was also used to examine whether developmental abnormalities in the embryos are similar to those found in a homozygous genotype.

Research, Embryology, Animal embryology, Lethal mutation

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Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo

Article Abstract:

Genes from mouse embryos were hybridized with various probes to determine the role of mouse hedgehog genes in late embryogenesis. The genes were expressed in unique locations and showed no overlap in functions. These results suggest that hedgehog genes have their own signaling activity and play a role in various morphogenetic processes within the cell. Evidence also suggests that hedgehog genes are conserved in vertebrate development.

Morphogenesis, Cell interaction, Cell interactions

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