Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light
Article Abstract:
A monomeric photoactivatable fluorescent protein (PAFP), Dandra, derived from octocoral Dendronephthya sp. and capable of 1,000- to 4,500-fold photoconversion from green to red fluorescent states in response to either visible blue or ultra violet light is reported. Dendra represents the first PAFP, which is simultaneously monomeric, efficiently matured at 37 degree Celsius, demonstrating high photostability of the activated state.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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Photoswitchable cyan fluorescent protein for protein tracking
Article Abstract:
A dual-color monomeric protein, photoswitchable cyan fluorescent protein (PS-CFP) that is used for protein tracking is described. PS-CFP is used as a photoswitchable tag to analyze trafficking of human dopamine transporter in living cells and at moderate excitation intensities, it can be used as a pH-stable cyan label for protein tagging and fluorescence resonance energy transfer applications.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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A genetically encoded photosensitizer
Article Abstract:
A genetically encoded photosensitizer called KillerRed, developed from the hydrozoan chromoprotein anm2Cp, a homolog of green fluorescent protein (GFP) is reported. The utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase C&1 pleckstrin homology domain is demonstrated.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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