Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
Article Abstract:
The study of the growth of commonly used laboratory strains of Escherichia coli revealed that they secrete YebF, a small soluble endogenous protein into the medium, which challenges the prevailing view that laboratory strain do not secrete proteins to the medium. The study demonstrates the efficient secretion of 'passenger' proteins linked to the carboxyl end of YebF.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
Article Abstract:
An attempt is made to show that the technology that makes it possible to genetically encode unnatural amino acids can be used to efficiently and site-specifically incorporate p-iodo-L-phenylalanine (iodoPhe) into proteins in response to an amber TAG codon.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli. Avimers hold their own
- Abstracts: ScanBalt coordinates intellectual property in Northern Europe. Rising biotech appetite in the east
- Abstracts: Evidence and anecdotes: An analysis of human gene patenting controversies
- Abstracts: High-throughput identification of transcription start sites, conserved promoter motifs and predicted regulons