Reversible site-selective labeling of membrane proteins in live cells
Article Abstract:
A generic method comprising of molecular probes, created from chromophore and metal-ion-chelating nitrilotriacetate (NTA) moiety is used for rapid, reversible and site-selective labeling of proteins in live cells. NTA probes can be applied for in vitro and in vivo investigations of molecular interactions and can facilitate the qualitative analysis of the biochemical networks that control and maintain the cellular functions.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues
Article Abstract:
Membrane protein assemblies are difficult to study due to their sensitivity to solubilizing detergents. Research is done on a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity and the study of protein interactions in complex tissues is extensively examined.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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Membrane proteins up for grabs
Article Abstract:
A simple algorithm is designed for the integration of transmembrane helices and bound with target helix. The results show implications for protein design and may be useful compound for drugs.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2007
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