Diffusion of lipid-like single-molecule fluorophores in the cell membrane
Article Abstract:
The photophysical and diffusive behaviors of the dicyanomethylenedihydrofuran (DCDHF) lipid analogues in membranes are described and are found to be competitive with the well-known lipid probe. It is concluded that several members of a new class of single-molecule emitters could serve as useful lipid analogues in the plasma membrane of Chinese hamster ovary (CHO) cells, with sufficient brightness and stability to allow tracking of single-molecule diffusion.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopy
Article Abstract:
Hydrophobic interactions play a major role in binding non-native substrate proteins in the central cavity of the bacterial chaperonin GroEL. The sequence of local conformational changes by which GroEL and its cofactor GroES assist in protein folding can be explored using the polarity-sensitive fluorescence probe Nile Red.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Bulk and single-molecule characterization of an improved molecular beacon utilizing H-dimer excitonic behavior
Article Abstract:
A pair of fluorophores in the new dicyanomethylenedihydrofuran (DCDHF) dye family was used to design and characterize a new fluorescent probe for nucleic acid detection, referred as a self-quenched intramolecular dimer (SQuID) molecular beacon (MB).
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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