Adipocyte insulin responsiveness in female Sprague-Dawley rats fed a low fat diet containing a fat-mimetic carbohydrate

Article Abstract:

The physiologic response to insulin and receptor number of rat adipocytes were studied using dietary increments of variable fat levels and fat-mimetic carbohydrates. Adipocyte glucose oxidation analysis showed that a high fat diet increased adipocyte synthesis. Total energy intake in a high fat diet remained constant. Weight gain was attributed to increased protein and glycogen liver stores. Insulin did not alter overall lipid synthesis although levels were increased proportional to fat intake. Less receptors caused subsequent reduction in insulin sensitivity although physical factors inherent to the rat strain should be considered.

Low-fat diet, Low fat diet, Dietary fat, Insulin, Carbohydrates in the body

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Regulation of hepatic transthyretin messenger RNA levels during moderate protein and food restriction in rats

Article Abstract:

Transthyretin is a nutritional marker that is used to detect moderate protein-energy malnutrition and monitor the effectiveness of nutritional support. An investigation was conducted to determine the primary factor involved in decreasing serum transthyretin levels and the relationship between these serum levels and hepatic transthyretin mRNA expression. The results showed that serum transthyretin levels are determined primarily by energy intake. There was a partial correlation between hepatic transthyretin mRNA expression and serum transthyretin levels.

Gene expression, Messenger RNA, Blood proteins

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Regulation of hepatic lipogenic enzyme gene expression by diet quantity in rats fed a fat-free, high carbohydrate diet

Article Abstract:

The influence of carbohydrate intake on lipogenic enzyme gene transcription in rat liver was examined by measuring translational rates and enzymatic activities after refeeding fasted rats. Results showed that messenger RNA transcription increases with increasing food intake. Evaluation of enzymatic activities also showed increasing fatty acid and triglyceride synthesis in rats fed more than 70% of the ad libitum diet. These indicate that high carbohydrate levels trigger the expression of the genes coding for lipogenic enzymes.

Genetic regulation, Genetic transcription, Transcription (Genetics), Lipids, Lipid synthesis

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