Application of models to determination of nutrient requirements: experimental techniques employing tracers

Article Abstract:

Techniques in developing a compartmental model of nutrient kinetics in the entire body is presented. The design of a tracer experiment including the administration strategy, use of two or more tracers, sampling technique, measurement methodology and duration of the experiment is discussed. In developing mathematical models, it is important to have pilot models and experiments in order to test the merits of the research and circumvent possible difficulties that may arise in the course of the study.

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Application of models to the determination of nutrient requirements: an overview

Article Abstract:

The use of mathematical modeling in nutrition research is an important method in explaining interrelationships between parameters and existing processes. One benefit of using mathematical models, particularly in the field of nutrition, is that it guides the researcher in considering even the smallest detail of experimental and analytical factors and processes. The use of modeling in the prediction of growth requirements for vitamin B-6 is discussed.

Models

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Metabolism of (14C)- and (32-P)pyridoxal 5'-phosphate and (3H) pyridoxal aministertered intravenously to pigs and goats

Article Abstract:

The kinetics of in vivo vitamin B6 metabolism was studied in pigs and goats. Pigs were used because of their similarity to human biochemical processes. Goats were used to investigate lactation, because their milk has high pyridoxal phosphate levels. Radioactively labeled pyridoxal phosphate and pyridoxal was monitored after intravenous administration. Results are presented.

Physiological aspects, Swine, Goats, Vitamin metabolism, Vitamin B6, Pyridoxine

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