Energy restriction and testosterone implants alter ornithine decarboxylase gene expression in kidney of female rats

Article Abstract:

Studies were made on the effects of testosterone and energy restriction on ornithine decarboxylase (ODC) enzyme activity and mRNA level in tissues of female rats. A one-week testosterone implantation, without diet restriction, raised ODC activity and mRNA level in 14-week-old female rats. A 14-week diet restriction, with or without testosterone implants, further stimulated renal ODC activity while leaving the mRNA level unaffected. Thus, diet restriction, not testosterone level, seems to be the cause of increased renal ODC activity stimulation, perhaps by heightening the rats' androgen sensitivity.

Testosterone, Ornithine decarboxylase

---

Eukaryotic transcription factors: identification, characterization and functions

Article Abstract:

DNA binding assays and purification and cloning are some of the most common methods used for identifying and characterizing eukaryotic transcription factors in studies on gene expression. DNA binding assays consist primarily of two techniques, the electrophoretic mobility shift assay and the DNase I protection assay. Variations of these two binding assay methods include methylation interference, ultraviolet crosslinking and Southwestern blotting. Purification and cloning approaches include biochemical purification. in situ detection and yeast one-hybrid selection system.

Eukaryotic cells, Cells (Biology), Eukaryotes

---

mRNA quantification techniques: considerations for experimental design and application

Article Abstract:

Several methods for identifying and and quantifying mRNA species coding of specific proteins have been developed for studies on the regulation of gene expression. The three most widely used techniques are Northern blot analysis, ribonuclease protection assay and quantitative revers transcriptase-coupled polymerase chain reaction. The advantages and disadvantages of each methods as well as their applications and limitations are described.

Usage, Polymerase chain reaction, Messenger RNA

Similar abstracts:

• Abstracts: Developmental stages and energy restriction affect cellular oncogene expression in tissues of female rats
• Abstracts: Dietary alpha-linoleic acid deficiency and early uterine development in female rats. Dietary calcium modifies concentrations of lead and other metals and renal calbindin in rats
• Abstracts: Chefs' salads. Less is more: dining on the light side main-course salads. Crowd-pleasing salads
• Abstracts: Lymph chylomicron composition and size are modified by level of intestinally infused cholesterol and triglyceride source in rats

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.