Confirmatory testing of Chlamydia trachomatis Syva enzyme immunoassay gray zone specimens by Syva direct fluorescent antibody test

Article Abstract:

More than 40% of endocervical samples screened by enzyme immunoassay for Chlamydia trachomatis that were classified as falling into a gray zone initially, tested positive on repeat screening with direct fluorescent antibody testing. The first screening was done using the Syva Microtrak enzyme immunoassay test. Specimens which fell into the gray zone category, that is they might be positive, were centrifuged and re-suspended. The samples were then evaluated by Syva Microtrak direct fluorescent antibody testing. After the first round of confirmation testing was completed, a policy of confirmation testing for all gray zone specimens was instituted. During a 12-month period, 42.9% of 175 re-tested gray zone samples were re-classified as positive. Thus, gray zone samples should not be classified as negative for chlamydia trachomatis until subjected to repeat screening.

Usage, Fluorescent antibody technique

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Confirmation of the Syva Micro Trak enzyme immunoassay for Chlamydia trachomatis by Syva Direct Fluorescent antibody test

Article Abstract:

A popular commercial screening test for Chlamydia appears to require follow-up testing on women testing positive to reduce the number of false-positive tests, but any one follow-up test may produce a false-negative result. Among 136 cervical secretion specimens originally testing positive for Chlamydia, 20, or 15%, tested negative on a second type of test. Of these 20, 11 tested negative on both of yet two other types of test while the rest tested positive on one or both of these other tests. Therefore, follow-up testing by two different tests reduced the false-positive rate from 15% to 8%.

Testing, Chlamydia trachomatis

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Use of DNA purification kits for polymerase chain reaction testing of Gen-Probe Chlamydia trachomatis PACE 2 specimens

Article Abstract:

The polymerase chain reaction (PCR) test can be used to confirm results of chlamydia testing using Gen-Probe PACE 2, a nucleic acid hybridization screening test for the sexually transmitted disease. Confirmational testing improves the accuracy of diagnostic testing, and allows less expensive testing methods to be used for disease screening. Researchers tested several DNA purification techniques for use with the Roche AMPLICOR PCR test for the Chlamydia trachomatis bacteria. The Microcon 100 kit by Amicon produced the most consistent DNA purification.

Methods, Product/Service Evaluation, Nucleic acid hybridization, Polymerase chain reaction, Diagnosis, Noninvasive, Noninvasive diagnosis, Amplicor (Medical test kit)

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