Detection of human papillomaviruses in cervicovaginal cells using polymerase chain reaction

Article Abstract:

The presence of human papillomaviruses (HPV) is readily detectable by a number of DNA hybridization and amplification techniques, the most sensitive of which is the polymerase chain reaction (PCR). The number of positive findings obtained with the PCR exceeds that obtained by other techniques. Researchers applied PCR to the detection of HPV in gynecological specimens. Cervicovaginal washings were collected from 102 healthy female subjects and 12 patients with class III cervical intraepithelial neoplasia (CIN). Washings from 107 HPV-positive and 48 HPV-negative women were retested by the PCR technique 5 to 7 days after the first test. HPV DNA was found in 43 of the 102 healthy women, and in all 12 of the CIN-positive patients. Of the 107 women who initially tested positive for HPV, 81 tested positive for HPV, but 26 had no detectable HPV DNA when retested by the PCR. In addition, 9 of the 48 HPV-negative specimens were positive for HPV when tested again; 5 of the 9 specimens were subsequently diagnosed to contain condylomata acuminata (venereal warts). The data suggest that women suspected of HPV infection who have negative findings should be retested by PCR and that even positive results on initial testing may be rechecked to obtain confirmation of HPV diagnosis. (Consumer Summary produced by Reliance Medical Information, Inc.)

Usage, Papillomaviruses, Papillomavirus, Identification and classification, Polymerase chain reaction, Vaginal diseases, Cervix diseases

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Recurrent cervical carcinoma after primary radical surgery

Article Abstract:

Women who have a relapse of cervical cancer after surgical treatment may benefit from chemotherapy and/or radiation treatment. In a study of 177 patients who relapsed after surgery, those who received chemotherapy or radiotherapy had a better prognosis than those who did not.

Cancer, Cancer recurrence

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Value of human papillomavirus deoxyribonucleic acid testing after conization in the prediction of residual disease in the subsequent hysterectomy specimen

Article Abstract:

A test that detects DNA of the human papillomavirus can be used to determine whether women with cervical cancer have any cancer remaining after cervical conization. Cervical conization is a surgical procedure that removes the cancerous tissue.

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