Measuring the frequency of mouse and human cytotoxic T cells by the Lysispot assay: independent regulation of cytokine secretion and short-term killing
Article Abstract:
Research demonstrates that the Lysispot assay in which cytokine-secreting and cytotoxic cells can be simultaneously enumerated is a powerful ex vivo analysis for measuring immune response during infection and autoimmunity.The assay consisting of antigen-specific, perforin-dependent Lysispot is combined with a cytokine Elispot to verify the independent regulation of cytotoxicity and interferon-gamma secretion.
Publication Name: Nature Medicine
Subject: Health
ISSN: 1078-8956
Year: 2003
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A quantitative assay for HIV DNA integration in vivo
Article Abstract:
This article examines techniques to help the virologist study how HIV viruses interact with DNA in vivo. A quantitative assay is described that analyzes how the virus integrates its DNA into the chromosomes of its host.
Publication Name: Nature Medicine
Subject: Health
ISSN: 1078-8956
Year: 2001
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A sensitive and high-throughput assay to detect low-abundance proteins in serum
Article Abstract:
A modular, facile amplification system, termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT) is created. The detection of Her2 from rodent and human sera is described.
Publication Name: Nature Medicine
Subject: Health
ISSN: 1078-8956
Year: 2006
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