Quantifying HIV-1 RNA using the polymerase chain reaction on cerebrospinal fluid and serum of seropositive individuals with and without neurologic abnormalities
Article Abstract:
Levels of HIV RNA in cerebrospinal fluid (CSF) may not be an accurate indicator of HIV neurologic disease (HND). Researchers took blood and cerebrospinal fluid samples from 97 HIV-positive men and 13 high-risk HIV-negative men. The polymerase chain reaction was used to measure the amount of HIV RNA in the samples. Forty-three of the HIV-positive men had signs and symptoms of HND. PCR detected HIV RNA in 96% of the blood samples from infected men but only 55% of the CSF samples. When the CSF samples were concentrated, HIV RNA was detected in 78% of the samples. Blood RNA levels were much higher in all of the men than CSF RNA levels. Higher blood RNA levels were associated with lower CD4 cell counts. High blood RNA levels were also associated with various measures of neurologic function. There was no association between CSF RNA levels and HND.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1995
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A single-dose study to assess the penetration of stavudine into human cerebrospinal fluid in adults
Article Abstract:
The AIDS drug stavudine appears to penetrate the blood-brain barrier and may be useful in treating neurologic complications of HIV infection. Researchers took cerebrospinal fluid (CSF) samples from 12 volunteers at various times after giving them a single dose of stavudine. Stavudine did not reach the CSF until two hours after the drug was taken. The drug reached levels in the CSF that should be adequate for treating HIV infection of the brain.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1998
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Failure to detect nelfinavir in the cerebrospinal fluid of HIV-1-infected patients with and without AIDS dementia complex
Article Abstract:
The AIDS drug nelfinavir appears to reduce viral levels in the cerebrospinal fluid (CSF) even though the drug does not penetrate the blood-brain barrier effectively. Researchers measured nelfinavir levels in CSF samples from six HIV patients after they began taking the drug along with other AIDS drugs. Four patients had AIDS dementia complex. Nelfinavir was not detected in any CSF samples but the amount of virus in CSF and blood decreased.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1999
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