A designed metal-binding protein with a novel fold
Article Abstract:
An all-beta protein was designed and synthesized based on the three beta- strands from eachof the two beta-sheets of the variable heavy domain of the immunoglobulin McPC603. The 61-residue protein, termed a minibody, retained the hypervariable H1 and H2 loops, which forms the site for creating functional centers. By introducing one histidine in H1 and two in H2, a metal-binding site was successfully engineered. The minibody loops are amenable to sequence changes and may also be used for the construction of peptide libraries.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Loose-patch recordings of single quanta at individual hippocampal synapses
Article Abstract:
Researchers have succeeded in detecting miniature excitatory postsynaptic currents ('minis') arising from spontaneous vesicle exocytosis at a single synaptic site. These were compared with minis identified simultaneously in the cell body. It was established that it is possible to accurately estimate the quantal properties of a single central synapse. Quantal variability at individual glutamatergic synapses is much shorter than that identified in population recordings.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity
Article Abstract:
Jun activation domain-binding protein 1 (JAB1), a coactivator of the c-Jun transcription factor, is present in the nucleus and the cytoplasm of cells. A fraction of JAB1 colocalizes with the alpha-L/beta-2 integrin LFA-1 at the cell membrane. Signalling through the LFA-1 integrin may influence c-Jun-prompted transcription.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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