Chromosome engineering in mice

Article Abstract:

Large deletions, duplications and inversions can be designed in embryonic stem cells in mice. The method involves the Cre protein, an enzyme that catalyzes the recombination between loxP sites. Consecutive targeting of loxP recombination substrates followed by recombination stimulated by Cre produces defined deficiencies. Deletions to the tune of 90 kb to 3-4 cM, which serve as a tool for screening of recessive mutations, are possible. The availability of mice with segmental haploidy will be useful in genetic screens which will help develop models of human chromosomal diseases.

Observations, Stem cells, Mice as laboratory animals, House mouse, Animal mutation

---

Are vertebrate exons scanned during splice-site selection?

Article Abstract:

Precursor RNAs containing duplicated sites within an exon were used to determine that pairwise recognition of splice sites occurs within exons rather than introns in vertebrates. A scanning mechanism for exons would be evidenced by the observation of depressed polyadenylation when a 5' splice is inserted in a 3'-terminal exon. Both in vitro and in vivo recognition of a 5' splice site located within a 3'-terminal exon correlated with depressed polyadenylation and ultraviolet crosslinking of a polyadenylation factor.

Vertebrates, Introns

---

A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in trandsgenic mice

Article Abstract:

A 250 kilobase yeast artificial chromosome carrying the mouse tyrosinase gene is transferred into the somatic cells of mice by pronuclear injection. The gene did not express itelf and did not undergo any major rearrangements during the process. Transgene expression was almost equal to endogenous gene expression. Copy number dependence and position independence were also exhibited. The technique could be used for other experiments involving gene transfers where correct gene expression is important.

Yeast fungi, Microbial biotechnology, Yeasts (Fungi), Genetically modified mice

Similar abstracts:

• Abstracts: A deadly inheritance. Germ-line transmission of a mutated p53 gene in a cancer-prone family with Li-Fraumeni syndrome
• Abstracts: Insulin-IGF2 region on chromosome 11p encodes a gene implicated in HLA-DR4-dependent diabetes susceptibility. Sweet mice, sugar daddies
• Abstracts: Insulin-IGF2 region on chromosome 11p encodes a gene implicated in HLA-DR4-dependent diabetes susceptibility. part 2
• Abstracts: Vesicle assembly in microtubes. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine
• Abstracts: Influence of dietary cation-anion balance during the dry period on the occurence of parturient paresis in cows fed excess calcium

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.