Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation
Article Abstract:
It is possible to prevent chromatin nucleating microtubules by inhibiting RCC1, the guanine-nucleotide-exchange factor for the GTPase protein Ran, from producing Ran-GTP by RanT24N. This can also be achieved by boosting the rate of reconversion of Ran-GTP generated by RCC1 activity back to the GDP-bound state by using excess ranBP1 and ranGAP. The activity of chromosome-associated RCC1 protein is needed for spindle formation. This research has provided a molecular basis for the impact of chromosomes on local microtubule growth seen in dividing plant cells and meiotic animal cells.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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RanGTP mediates nuclear pore complex assembly
Article Abstract:
RanGTP triggering distinct steps of nuclear pore complexes (NPC) assembly is demonstrated and a mechanism for the spatial restriction of NPC assembly to the surface of chromatin is suggested. NPC, assemble into the reforming nuclear envelope in a sequential process involving association of a subset of NPC proteins, nucleoporins, with chromatin followed by the formation of a closed nuclear envelope fenestrated by NPCs (super 2-7).
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2003
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Structural basis for nuclear import complex dissociation by RanGTP
Article Abstract:
The structure of full-length yeast importin-beta complexed with RanGTP, which provides a basis for understanding the crucial cargo release step of nuclear import, is investigated. An allosteric mechanism for nuclear import complex disassembly by RanGTP is suggested.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2005
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