Guilt-by-association goes global
Article Abstract:
Two different strategies have been used in a detailed analysis of yeast protein-protein interactions. With the array screening approach, it quickly becomes clear which locations give false-positive interactions. With the library screening approach, cells expressing the 6,000 different 'prey' molecules are not kept separate. The array method is more efficient, while with the library method cells in the 'prey' pool compete with each other during growth and mating, thus selecting against cell expression fusion products that retard either process. It has been possible to identify previously unknown interactions between proteins involved in the same biological process.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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Microtubule-motor activity of a yeast centromere-binding protein complex
Article Abstract:
The role of motor proteins in the chromosome segregation that takes place during cell division is now known in greater detail. The proteins assemble into a structure called the kinetochore, which controls the linking of chromosomes to the mitotic spindle microtubules. The kinetochore allows the CBF3 protein complex to attach the DNA to the microtubules. Moreover, the CBF3 complex includes a minus-end-directed microtubule-based motor protein. These motors probably join microtubules and chromosomes together during mitosis.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA
Article Abstract:
Eg2, a member of the Aurora family of serine/threonine protein kinases, is activated within 30 minutes following the addition of progesterone to oocytes. It phosphorylates CPEB, the cytoplasmic polyadenylation element binding factor, on Ser 174, which is required and sufficient to prompt the cytoplasmic polyadenylation and subsequent translational recruitment of c-mos messenger RNA. CPEB phosphorylation may enhance the binding of cleavage and polyadenylation specificity factor to the AAUAAA hexanucleotide.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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