Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by x-ray crystallography
Article Abstract:
X-ray crystallography was used to establish the three-dimensional structure of the lipase-procolipase complex at 3 angstrom resolution. The lipase-procolipase complex was co-crystallized with mixed micelles of phosphatidylcholine and bile salt. It was also shown that the phospholipid was allowed to bind with the enzyme's active site when the lid changed its conformation. The lid was characterized as a surface helix which covered the catalytic triad of lipase. Interaction between the open lid and procolipase form the lipid-water interface binding site.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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The atomic structure of the bluutongue virus core
Article Abstract:
Bluetongue virus (BTV) is a species of the Orbivirus within the Reoviradae group. X-ray crystallography has been used to determine the structure of the core particle of BTV. The atomic structure gives an insight into how around 1,000 protein components self-assemble using both the classical mechanism of quasi-equivalent contacts and geometrical quasi-equivalence.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
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