Cystic fibrosis in the mouse by targeted insertional mutagenesis
Article Abstract:
A new mouse model for cystic fibrosis (CF) has been devised that should be useful in trials of potential treatments for this deadly autosomal recessive disorder. The new model was produced through mutagenesis or the genetic disruption of the CF transmembrane conductance regulator gene in murine embryonic stem cells. The detection of the characteristic CF pathophysiology in the mice such as abnormal chloride ion transport verified the similarity of the model to human CF.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Magnetic bead capture of expressed sequences encoded within large genomic segments
Article Abstract:
Biotin-streptavidin magnetic bead technology can be used effectively to isolate complementary DNA (cDNAs) encoded in large genomic segments. Conventional methods are inefficient when the genomic interval is greater than several hundred thousand base pairs. Magnetic bead technology may be used to enrich cDNA encoded over very large segments such as the candidate gene for Huntington's disease.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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- Abstracts: Forest fires in the Brazilian Amazon. Conservation Threat of Increasing Fire Frequencies in the Western Ghats, India
- Abstracts: Mulling over mouse models. Cloning the Menkes disease gene
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- Abstracts: Court-appointed scientists provide technical expertise. Gene therapy research is spread across many disciplines and institutes at NIH