Regulation of apical transport in epithelial cells by a Gs class of heterotrimeric G protein
Article Abstract:
The role of heterotrimeric GTP-binding proteins (G proteins) in the polarized transport of proteins in the apical and basolateral domains of epithelial Madin-Darby Canine Kidney cells was investigated. The results showed that the Gs class of G proteins mediate the apical transport pathway while the Gi class mediates the basolateral pathway. The results show that the G proteins regulate the transport of proteins from the trans-Golgi network to the surface, probably through a mechanism that modulates the efficiency of sorting.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Thomas Kreis (1952-98): Cell biologist
Article Abstract:
Thomas Kreis was a leading cell biologist, entering cell biology in the 1970s. He was one of the first to study cellular function within the context of living cells, and was already microinjecting fluorescently labelled proteins into live cells during his PhD between 1978 and 1981. He was group leader in the cell biology programme at the European Molecular Biology Laboratory in Heidelberg, and moved to Geneva as a professor at the age of 40.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
N-glycans as apical sorting signals in epithelial cells
Article Abstract:
The N-glycans direct the sorting of secretory proteins from the apical rather than the basolateral surfaces in the epithelial Madin-Darby canine kidney (MDCK) cells. Non-glycosylated growth hormone (GH) is released from both the apical and basolateral layers of the MDCK cells, but glycosylated GH exhibits apical sorting. For secretory proteins, the carbohydrates are the apical targeting signals.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: BMP-4-responsive regulation of dorsal-ventral patterning by the homeobox protein Mix.1. Induction of the mammalian node requires Arkadia function in the extraembryonic lineages
- Abstracts: Retinoblastoma in transgenic mice. Ras signalling linked to the cell-cycle machinery by the retinoblastoma protein
- Abstracts: Beauty on the brain. Selection of exaggerated male traits by female aesthetic senses. Neural networks and olive oil
- Abstracts: Reduced number of ribosomal sites in bats: evidence for a mechanism to contain genome size. J. Knox Jones, Jr.: 1929-1992
- Abstracts: Conversion of lytic to persistent alphavirus infection by the bcl-2 cellular oncogene. part 2 Bax-independent inhibition of apoptosis by Bcl-x(sub l)