Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate

Article Abstract:

Phosphatidyl-inositol-4-5-bisphosphate (PtdInsP2) dissociates the head-tail interaction of a cytoskeletal protein, vinculin. PtdInsP2 exposes the talin- and actin-binding sites, induces a conformational change in vinculin and allows its association with other components of focal adhesions. The formation of focal adhesions and stress fibers depends on the GTP-binding protein Rho which is stimulated by serum. Rho increases the levels of PtdInsP2 and activates phosphatidyl-4-phosphate-5-OH kinase. Antibodies against PtdInsP2 suppress the formation of focal adhesions and stress fibers.

Physiological aspects, Protein kinases, G proteins, Binding sites (Biochemistry), Active sites (Biochemistry)

---

F-actin binding site masked by the intramolecular association of vinculin head and tail domains

Article Abstract:

The carboxy-terminal tail domain of the vinculin exhibits intramolecular association between the 30K tail and 95K head domains. This association masks the F-actin binding site in the tail domain of the carboxy-terminal. The F-actin binding site requires vinculin for attachment of myofibrils to sarcolemma and plasma membranes. The masking process and association between the tail and head domains also facilitate the attachment of myofibrils to plasma membranes and sarcolemma.

Sarcolemma

---

Cryptic sites in vinculin

Article Abstract:

Vinculin, a cytoskeletal protein found at cell adhesion sites, is capable of reacting with actin directly. Two researchers of the John Hopkins University School of Medicine, Baltimore, MA, have discovered that vinculin has an actin binding site, which is usually masked in the purified proteins. The binding sites are caused by the presence of contaminants within the vinculin.

Similar abstracts:

• Abstracts: Regulation of cancer cell migration and bone metastasis by RANKL . Stromal fibroblasts in cancer initiation and progression
• Abstracts: Amplification of chirality in two-dimensional enantiomorphous lattices. Enantioselective silyl protection of alcohols catalysed by an amino-acid-based small molecule
• Abstracts: Termination of asymmetric cell division and differentiation of stomata. Endothelial tubes assemble from intracellular vacuoles in vivo
• Abstracts: Crystal structure of a mammalian phosphoinositide-specific phospholipase C-delta. Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4
• Abstracts: Quantum nature of a strongly coupled single quantum dot-cavity system. Optical emission from a charge-tunable quantum ring

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.