A complex group I intron in Nectria galligena rDNA
Article Abstract:
M.A. Crockard et al. reported on a polymorphic insertion element of 363, 1185 or 1423 bp within the nuclear small subunit (SSU) rDNA of the ascomycete fungus Nectria galligena. The insertion site was mapped to location 1199, a site which is known to have nuclear group I introns in fungi. However, they did not allocate this insertion to any known class of insertion elements. An examination of the Nectria sequences reported by Crockard et al. showed that the smallest insertion has a typical group I intron structure. It is distantly related to the subgroup IC1 introns in nuclear rDNA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Neocallimastix frontalis enolase gene, enol: first report of an intron in an anaerobic fungus
Article Abstract:
The anaerobic rumen fungus Neocallimastix frontalis contains a single enolase gene, enol, interrupted by large intron. The G+C content of the enolase coding sequence is much higher than that of the intron coding sequence and the enzyme is probably a homodimer of the gene product. The amino acid sequence of enol is similar to the enolases of Saccharomyces cerevisiae and Candida albicans. The expression of the gene depends on the carbon source and the fungi mycellium grown on glucose has a higher enolase mRNA content than the mycellium grown on cellulose.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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A secreted aspartic proteinase from Glomerella cingulata: purification of the enzyme and molecular cloning of the cDNA
Article Abstract:
Ion exchange chromatography was used in the purification of the secreted aspartic proteinase from Glomerella cingulata (GcSAP). Some properties of the enzyme, molecular cloning of the cDNA and the gene encoding the enzyme were also described. Molecular cloning of the cDNA for GcSAP made the assessment of the role of the enzyme in pathogenicity by a targeted gene disruption possible.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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