A novel bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of flavescence doree in Vitis vinifera
Article Abstract:
Flavescence doree, is a grapevine disease caused by the bacterium 'Candidatus Phytoplasma vitis' and spread throughout the vineyards by a leafhopper, Scaphoideus titanus. A survey was conducted to characterize the bacterial microflora associated with S. titanus using length heterogeneity PCR and the results reveal that this endosymbiont and "Ca. Phytoplasma vitis" have a partially overlapping colonization pathway in the insect body, colocalizing in the fat body and salivary glands.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Comparison of different primer sets for use in automated ribosomal intergenic spacer analysis of complex bacterial communities
Article Abstract:
ITSF and ITSReub, constituting a new primer set designed for the amplification of the 16S-23S rRNA intergenic transcribed spacers, was compared with different primer sets previously proposed for automated ribosomal intergenic spacer analysis (ARISA) of complex bacterial communities. Results demonstrate that the ITSF/ITSReub primer set emphasizes the capacity of ARISA to powerfully explore microbial diversity and to create complex, easy-to-analyze molecular fingerprintings.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Diversity of bacillus anthracis strains in Georgia and of vaccine strains from the former Soviet Union
Article Abstract:
Multiple-locus (MLVA) and other molecular typing methods were used to investigate Bacillus anthracis diversity in Georgia and to characterize vaccine strains used in former Soviet Union. The MLVA-based data establishes genetic diversity of Bacillus anthracis in Southern Caucasus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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