A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores
Article Abstract:
Research was conducted to examine a new, sensitive, inexpensive and rapid bioassay for the detection of the emetic toxin of Bacillus cereus. The novel bioassay is based on the motility loss of boar spermatozoa upon 24 h of exposure to emetic B cereus strains extracts or contaminated food. Results indicate swollen mitochondria of the paralyzed spermatozoa but no epetion of cellular ATP or damage to plasma membrane integrity was observed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Bacteria, molds and toxins in water-damaged building materials
Article Abstract:
Water-damaged sites of indoor buildings are heavily colonized by Stachybotrys chartarum, Penicillium, Aspergillus, gram-negative bacteria, mycobacteria and endotoxins. Water extracts of gypsum liners from a water-damaged site show the presence of endotoxins and beta-D-glucan. The methanol extracts of water-damaged gypsum liner contain satratoxin, paralyzing the motility of boar spermatozoa at low concentrations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Semiautomated metabolic staining assay for Bacillus cereus emetic toxin
Article Abstract:
A study was conducted to determine bacterial strains that facilitate emetic toxin production. Bacterial strains of Baccilus cereus, Baccillus anthracis, Bacillus licheniformis, Baccillus mycoides, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus agri, Paenibacillus macerans and Paenibacillus polymyxa were evaluated. Results indicate that only strains of Bacillus cereus exhibit cytotoxicity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A novel means to develop strain-specific DNA probes for detecting bacteria in the environment. System for determining the relative fitness of multiple bacterial populations without using selective markets
- Abstracts: The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis. Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa
- Abstracts: Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe. Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii
- Abstracts: An alkane responsive expression system for the production of fine chemicals. Towards a biocatalyst for (S)-styrene oxide production: characterization of the styrene degradation pathway of Pseudomonas sp. strain VLB120
- Abstracts: Molecular cloning, nucleotide sequence, and expression in Escherichia coli of a hemolytic toxin (aerolysin) gene from Aeromonas trota