Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression

Article Abstract:

Analysis of promoter binding by the pRB and E2F and families in vivo indicates that distinct E2F proteins mediate repression and activation. A cross-linking approach in synchronized, living cells has been used to identify physiologically important E2F-responsive promoters and study their occupancy and histone acetylation state. The pattern of E2F and pRB-related polypeptides recruited to the promoters changes in a very dynamic way as the cells go from quiescence into G1 and S phase.

Carrier proteins, Transport proteins

---

Active repression and E2F inhibition by pRB are biochemically distinguishable

Article Abstract:

The retinoblastoma tumor suppressor protein can repress and inactivate independently the transcription factor E2F via targeting specific sets of transcription factors. Data indicate that the repression activity is associated with chromatin, distinguishing repression from the inactivation process.

Chromatin, Tumor suppressor genes, DNA binding proteins

---

Culprits in the degradation of cyclin E apprehended

Article Abstract:

The degradation of cyclin E, the Cullin genes and the Cullin/CDC53 family of proteins, two members in particular, Cul3 and Cul1, are discussed in this overview article. Data show that likely multiple pathways contribute to cyclin E turnover, at least in certain types of cells. The Cul1 pathway may be more general. Unsolved questions are discussed. Analysis of cell cycle regulators will help in finding important ubiquitination pathways.

Statistical Data Included, DNA, Cellular signal transduction, Mitosis, Phosphorylation, Trophoblast, Trophoblasts, Ubiquitin, Ligases

Similar abstracts:

• Abstracts: Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression
• Abstracts: High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation
• Abstracts: A specific protein-protein interaction accounts for the in vivo substrate selectivity of Ptp3 towards the Fus3 MAP kinase
• Abstracts: BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
• Abstracts: Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.