Binding of germ cells to mutant Sl(super d) Sertoli cells is defective and is rescued by expression of the transmembrane form of the c-kit ligand
Article Abstract:
A study was conducted on the effect of the lack of the c-kit ligand (KL) in its transmembrane form on the interaction between Sertoli and germ cells using Sertoli cells obtained from Sl/Sl(super d) mutant mice. The expression of mRNAs coding for both soluble and transmembrane KL forms were found to be developmentally regulated in the testis. In addition, results showed that mebrane-bound KL was capable of mediating Sertoli cell-germ cell adhesion and that mutant Sl(super d) Sertoli cells were incapable of binding germ cells. This was remedied, however, by introducing plasmid expressing the transmembrane KL form.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1993
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A novel murine zinc finger gene mapped within the tw18 deletion region expresses in germ cells and embryonic nervous system
Article Abstract:
The isolation of NTfin12, a novel zinc finger gene belonging to the C2H2-Kruppel-type gene family, from a newborn mouse testis cDNA library is reported. Zinc finger consensus motif probes were used in the isolation. The NTfin12 mRNA was expressed during the meiotic prophase of spermatogenesis and in embryogenesis. The findings suggest a role for NTfin12 during germ cell development and specification of a subpopulation of neuroectodermal cells. Furthermore, genetic linkage analyses revealed that the NTfin12 locus mapped to the deletion region of the tw18 haplotype on mouse chromosome 17.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1993
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The gene encoding murine alpha-1,3-galactosyltransferase is expressed in female germ cells but not in male germ cells
Article Abstract:
The alpha-1,3-galactosyltransferase (alpha-3-GT) gene is exclusively expressed in female germ cells as understood by the model of sperm-zona binding. For a functional sperm binding determinant on zona pellucida glycoprotein 3, a nonreducing terminal alpha-galactosyl residue is necessary. In male germ cells the expression of alpha-3-GT is restricted to avoid sperm-sperm association.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
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