Cdk2 kinase is required for entry into mitosis as a positive regulator of Cdc2-cyclin B kinase activity
Article Abstract:
The protein cyclin-dependent kinase 2 (Cdk2) begins the process of mitosis by regulating the complexation of Cdc2 protein kinase with B cyclins. When Cdk2 was inhibited by a specific inhibitor, mitosis is halted and a concentration of Cdc2-cyclin b complexes is observed. This effect was readily reversed by either the addition of Cdk2-cyclin E or the addition of cyclin B. This pathway serves as a controlling point for preventing early mitosis while DNA synthesis is still occurring in the cell.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
P25(super rum1) order S phase and mitosis by acting as an inhibitor of the p34(super cdc2) mitotic kinase
Article Abstract:
The yeast cyclin-dependent kinase inhibitor p25(super rum1) controls the cell cycle by suppressing the activity of the p34(super cdc2)/p56(super cdc13) mitotic kinase. The protein, encoded by the rum gene, inhibits mitosis of cells present in the G1 and G2/M phase of the cell cycle. Premature mitosis of cells in the G1 phase is lethal. Overexpression of the rum gene causes repeated DNA replication by removing the block on the S phase. The protein also determines the length of the G1 phase.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Checkpoint pathways come of age
Article Abstract:
Studies have shown how the cell cycle checkpoint control that prevents mitosis when DNA is damaged, interacts with cell cycle regulators to stop the onset of mitosis. It is suggested that there is a common molecular mechanism underlying such control. It is likely that fission yeast is a suitable model system for further characterization of the checkpoint pathway and the development of suitable assays and screening procedures.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A UV-induced mutation in neurospora that affects translational regulation in response to arginine. Spontaneous mutation during the sexual cycle of Neurospora crassa
- Abstracts: The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease
- Abstracts: A non-essential glutamyl aminopeptidase is required for optimal growth of Lactococcus lactis MG1363 in milk. Molecular analysis of the regulation of nisin immunity
- Abstracts: Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces
- Abstracts: Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays