Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp

Article Abstract:

The gyrase B (gyrB) gene was examined as a molecular diagnostic probe seeking to differentiate Vibrio parahaemolyticus from closely related species. GyrB genes from V. parahaemolyticus, considered to be the common cause of diarrhea associated with the consumption of fishery products in the summer, and from the closely related V. alginolyticus were amplified, cloned and sequenced. A method that specifically detects the target organism was developed and tested in shrimp samples.

Research, Nucleotide sequence, Base sequence, Cloning, Pathogenic microorganisms, Gene amplification, Shrimps, Shrimps (Animals)

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Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes

Article Abstract:

A four-color multiplexed TaqMan probe-based real-time PCR assay for detection of total and pathogenic Vibrio parahaemolyticus in oysters was developed and optimized. The assay specifically and sensitively detected total and pathogenic Vibrio parahaemolyticus and was expected to provide a rapid and reliable alternative to conventional detection methods by reducing the analysis time and obviating the need for multiple assays.

Polymerase chain reaction, Structure, DNA microarrays

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Real -time reverse transcription-PCR for transcriptional expression analysis of virulence and housekeeping genes in viable but nonculturable Vibrio parahaemolyticus after recovery of culturability

Article Abstract:

A real-time reverse transcription-PCR method is developed to determine whether the recovery of culturability of viable but nonculturable (VBNC) Vibrio parahaemolyticus induced the expression of virulence genes coding. The results demonstrate that the recovery of culturability of VBNC cells of pathogenic V. parahaemolyticus is restricted to regrowth, without correlation with the induction of virulence gene expression.

Physiological aspects, Gene expression

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