Cosecretion of chaperones and low-molecular-size medium additives increases the yield of recombinant disulfide-bridged proteins
Article Abstract:
Research has been conducted on the native tissue-type plasminogen activator variant. The improvement of the activator variant's yield in the Escherichia coli periplasm has been carried out and described.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
gly gene cloning and expression and purification of glycinecin A, a bacteriocin produced by Xanthomonas campestris pv. glycines 8ra
Article Abstract:
Research has been conducted on the glycinecin A, a bacteriocin produced by Xanthomonas campestris pv. glycines. The cloning and expressing of the genes encoding this glycinecin has been carried out in Escherichia coli and the results are presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Cloning of a nitrilase gene from the cyanobacterium Synechocystis sp. strain PCC6803 and heterologous expression and characterization of the encoded protein
Article Abstract:
The authors have investigated the gene which encodes nitrilase and which has been discovered in photosynthetic cyanobacterium Synechocystis sp. strain PCC6803 genome sequence. The gene has been amplified via PCR and cloned into the expression vector, and the encoded protein heterologously expressed in Escherichia coli has demonstrated that the rcombinant strains cenvert benzonitrile into benzolate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Molecular characterization of laccase genes from the basidiomycete Corpinus cinereus and heterologous expression of the laccase Lcc1
- Abstracts: The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and characterization of the purC and purL genes
- Abstracts: Controlled autolysis and enzyme release in a recombinant lactococcal strain expressing the metalloendopeptidase enterolysin A
- Abstracts: Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in diary heifers
- Abstracts: Group A rotavirus in sewage samples from Barcelona and Cairo: emergence of unusual genotypes. Potential role of fomites in the vehicular transmission of human astroviruses