Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay

Article Abstract:

Many strains of the bacterium Prevotella intermedia and related species have the ability to attach to collagen. The collagen-binding activity, measurable by a microtiter plate adherence assay, appears to provide a mechanism for the cells to invade and attach to tissues, and to help in colonization. EDTA, type I and type IV collagen, fibrinogen, fibronectin and denatured collagen suppress the binding capacity. Pretreatment by exposing cells to 60 degrees C for 30 min or to proteinase K also suppresses the binding.

Causes of, Growth, Bacteria, Pathogenic, Pathogenic bacteria, Periodontal disease, Periodontal diseases

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Characterization of hgpA, a gene encoding a haemoglobin/haemoglobin-haptoglobin-binding protein of Haemophilus influenzae

Article Abstract:

A DNA fragment cloned from Haemophilus influenzae that encodes an approximately 120 kDa protein expressing haemoglobin-binding activity in Escherichia coli has been characterized. The complete nucleotide sequence of the cloned DNA fragment, hgpA, was determined and analyzed using partial sequence analysis. Results show that the gene shares a significant homology with other bacterial haem- and iron-utilization proteins. In addition, seven regions typical of TonB-dependent proteins were identified.

Genetic aspects, Carrier proteins, Transport proteins, Hemoglobin, Hemoglobins, Identification and classification, Bacterial proteins, Genetic code, Hemophilus influenzae, Haemophilus influenzae, Haptoglobin

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Floc stability and adhesion of green-fluorescent-protein-marked bacteria to flocs in activated sludge

Article Abstract:

The green fluorescent protein (GFP) was employed as a cellular marker to examine bacterial adhesion to activated sludge flocs in situ in sludge liquor. Cell surface hydrophobicity (CSH) was observed to be a significant parameter that determined the relative bacterial adhesion potential. A high value of CSH resulted in a high number of attached cells. However, the absolute adhesion of two test bacteria to various sludge flocs varied and cannot be explained by the floc characteristics.

Sewage sludge, Flocculants

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