Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
Article Abstract:
Flow cytometric analysis utilizing nucleic acid dyes, SYTO-13, TOTO-1 and YOYO-1, of Escherichia coli and marine prokaryotic populations was conducted. Histograms taken after treatment with either ribonuclease or deoxyribonuclease reveal that these nucleic acid dyes preferentially stain DNA in marine prokaryotes but stains both RNA and DNA in E. coli. The implications of these results in bacterial nucleic acid research is discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Spatial and temporal deposition of Hyphomonas strain VP-6 capsules involved in biofilm formation
Article Abstract:
Research was conducted to examine the spatial and temporal deposition of Hyphomonas strain VP-6 capsules involved in biofilm formation. The objective was to show that its spatial arrangement and synthesis are very different from those of MHS-3. Results based on the polarity and temporality of VP-6 extracellular polymeric substances expression substantially differ from the properties of Hyphomonas strain MHS-3 EPS expression.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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