Complementation of a Clostridium perfringens spo0A mutant with wild-type spo0A from other Clostridium species

Article Abstract:

Clostridium perfringens spo0A mutant IH101 is complemented with wild-type spo0A from four different Clostridium species, so as to evaluate whether C. perfringens can be used as a model organism for studying the sporulation process in other clostridia. Wild-type spo0A from C. acetobutylicum and C. tetani restores sporulation and enterotoxin production in IH101, while the inability of C. botulinum and C. difficile to complement spore formation in IH101 might be due to the low levels of spo0A transcription and Spo0A production.

Physiological aspects, Gene mutations, Gene mutation, Environmental aspects, Genetic aspects, Clostridium, Cell division

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Competitive fitness of Listeria monocytogenes serotype 1/2a and 4b strains in mixed cultures with and without food in the U.S. Food and Drug Administration enrichment protocol

Article Abstract:

The comparison of three strains of L. monocytogenes serotype 4b and five strains of serotype 1/2a in direct competition with each other in two-strain mixed cultures is presented by using the food and drug administration (FDA) bacterial analytical manual (BAM) enrichment protocol. It was observed that the differences in strain fitness that occurred were not attributable to serotype or genetic lineage.

United States, Drugs, In-Vitro Diagnostic Substance Manufacturing, Bacterial Culture Products, Listeria monocytogenes, Bacteriology, Bacterial cultures

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