In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase

Article Abstract:

A study was conducted to demonstrate that engineered polyhydroxyalkanoate (PHA) synthases could be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface. It demonstrated that protein engineering of PHA synthase enables the manufacture of PHA granules with covalently attached enzymes, suggesting an application in recycling of biocatalysts, such as in fine-chemical production.

Bacterial cell walls, Microbiological chemistry, Polyhydroxyalkanoates

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Mutations to toluene-4-monooxygenase that alter regiospecificity of indole oxidation and lead to production of novel indigoid pigments

Article Abstract:

Broad-substrate-range monooxygenase enzymes, including toluene-4-monooxygenase (T4MO), can catalyze the oxidation of indole. It is demonstrated that modification of the tmoA active site can change the products produced by the enzyme and lead to the production of novel pigments and other indole oxidation products with potential commercial and medical utility.

Petrochemical Manufacturing, Toluene, Oxidation-reduction reaction, Oxidation-reduction reactions, Indole, Indoles

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Biotransformation of N-Nitrosodimethylamine by Pseudomonas mendocina KR1

Article Abstract:

The pathway(s) of N-Nitrosodimethylamine (NDMA) biotransformation by Pseudomonas mendocina, a strain that possesses toluene-4-monooxygenase KR1 was elucidated. It was found that strain KR1 did not grow on NDMA or mineralize significant quantities of the compound to carbon dioxide suggesting that the degradation process is cometabolic.

New Jersey, Industrial organic chemicals, not elsewhere classified, Analytical Laboratory Instrument Manufacturing, Analytical instruments, Methylamines, Ion Mass Spectroscopy, Analysis, Physiological aspects, Pseudomonas, Mass spectrometry, Decomposition (Chemistry)

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