DNA markers for differentiating isolates of Paecilomyces lilacinus

Article Abstract:

Randomly primed PCR and tRNA-PCR using consensus primers help evaluate the genetic variability among Paecilomyces lilacinus isolates from soil, nematodes or insects. The genotypes exhibit a high degree of variability. Cluster or multivariate analysis does not reveal any well-defined phenetic groups or relationship with the host or geographical origin. A subsample of 12 Paecilomyces lilacinus isolates, two Paecilomyces fumosoroseus isolates, one Paecilomyces amoenoroseus isolate and three Paecilomyces fasinosus isolates subjected to PCR with four consensus tRNA gene primer pairs exhibits the presence of a monophyletic group that contained most of the Paecilomyces lilacinus isolates.

Research, Phylogeny, Variation (Biology), Soil fungi

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tRNA intergenic spacers reveal polymorphism diagnostic for Xanthomonas albilineans

Article Abstract:

Polymerase chain reaction (PCR) primers, based on tRNA genes and polymorphism of intergenic areas, effectively identify the gram-negative pathogenic bacteria Xanthomonas albilineans in sugarcane samples. The use of primer pairs, 5'-3' tRNA(super ala) and 3'-5' tRNA(super ile), produces a PCR product characteristic of X. albilineans. The primers fail to form a product with other bacterial species. A nested PCR reaction based on the position of the tRNA(super ala)-tRNA(super ile) area inside the gene spacer increases the accuracy of the test.

Gram-negative bacteria, Identification and classification, Polymerase chain reaction, Genetic polymorphisms

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A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs

Article Abstract:

The use of scanning electron micrographs The use of scanning electron micrographs helped researchers determine that lytic enzymes and serine protease could help the parasitic Paecilomyces lilacinus fungus infect Meloidogyne spp. nematodes. The research involved putting the fungus on liquid mineral salts with substrates and purifying the resulting protease with affinity chromatography. Immature eggs were most susceptible to the invading protease, while young eggs and hatched larvae have better resistance.

Proteases, Nematoda, Nematodes, Scanning electron microscopes

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