ATP can be dispensable for prespliceosome formation in yeast

Article Abstract:

Evidence for a regulatory role of CUS2, a U2 suppressor protein, and a U2 snRNA structure in prespliceosome assembly has been found. ATP may only stimulate the rate of prespliceosome formation with CUS2 and U2 snRNA control. CUS2 brokers an interaction between PRP5 and the U2 snRNP that depends on the right U2 RNA structure. CUS2 seems to help couple PRP5 function to ATP-dependent steps in prespliceosome formation.

Physiological aspects, Binding sites (Biochemistry), Active sites (Biochemistry), Adenosine triphosphate, Messenger RNA, ATP

---

Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase

Article Abstract:

MUD2, the yeast homolog of U2AF65, and its deletion are discussed. MUD2 can bypass the essential functions for Sub2, a spliceosomal ATPase. Biochemical and genetic analyses suggest Sub2 may have more than one role in the splicing pathway before prespliceosome (PS) formation and also after. Sub2 may be not necessary when the likely protein target is not present.

Cellular signal transduction, Adenosine triphosphatase

---

Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice

Article Abstract:

RNA polymerase II coactivator subunit Srb7 in mice is discussed. Its expression is found to be ubiquitous and it is required for cell viability, based on results of its disruption in embryonic stem cells. The Srb7 (ital) gene is single copy. The yeast Srb/Med compex is involved in global gene expression and is essential. Srb7 is an Srb/Med complex protein with sequence and function much conserved from yeast to human. It seems Srb7-containing polymerase complexes are in most tissues with essential roles in expression of protein-coding genes.

Mice, Mice (Rodents), RNA polymerases, Embryology, Experimental, Embryological research, Stem cells

Similar abstracts:

• Abstracts: Stimulation of polyketide metabolism in Streptomyces fradiae by tylosin and its glycosylated precursors. The mycarose-biosynthetic genes of Streptomyces fradiae, producer of tylosin
• Abstracts: Cholesterol modification of sonic hedgehog is required for long-range signaling activity and effective modulation of signaling by Ptc1
• Abstracts: Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity. Identification and disruption of an Arabidopsis zinc finger gene controlling seed germination
• Abstracts: Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression
• Abstracts: NEUROGENIN1 and NEUROGENIN2 control two distinct waves of neurogenesis in developing dorsal root ganglia. A role for neural determination genes in specifying the dorsoventral identity of telecephalic neurons

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.